Hi Fred,
I used to worked with a protein which would not bind to the Qiagen
NiNTA (really *NOT*) but beautifully to IDA resin (e.g. Amersham
Pharmacia).
This was under non-denaturing conditions, though, and I don't know whether
this applies to the denatured state. It may be worth a try.
Tim
--
Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
GPG Key ID = A46BEE1A
On Tue, 27 Jan 2009, Fred wrote:
Hi ccp4 list,
I am trying to purify a his-tag protein by metal affinity chromatography. The
protein was expressed in inclusion bodies and its his-tag doesn't bind the
Qiagen Ni resin in denatured conditions (urea 8M or GndHCl 6M). Playing with
NaCl and detergents didn't help much.
Any help is appreciated.
Fred
