Dear Jacob, You can find several protocols for Se-Met labeling at:
http://www.doe-mbi.ucla.edu/local/protocols Good Luck, Art > Dear Crystallographers, > > I have been using an E coli strain called Tuner (DE3) pLacI, which is not > auxotrophic for methionine, nor is there an auxotrophic analogue > available. > It may be that my protein can be expressed in other strains, but this one > works well right now, and it is usually better to stay with what works > well. > Has anybody tried growing semet protein in non-auxotrophic strains, or are > there tricks to using non-auxotrophs for semet protein? > > Thanks, > > Jacob > > ******************************************* > Jacob Pearson Keller > Northwestern University > Medical Scientist Training Program > Dallos Laboratory > F. Searle 1-240 > 2240 Campus Drive > Evanston IL 60208 > lab: 847.491.2438 > cel: 773.608.9185 > email: [EMAIL PROTECTED] > ******************************************* > Arthur Laganowsky, M.S. David Eisenberg Laboratory University of California, Los Angeles Chemistry and Biochemistry Graduate Student 611 Charles E. Young Drive East 219 Boyer Los Angeles, California 90095 USA
