Hi Jacob,
you can express SeMet labeled protein in almost any E.coli expression
strain if you use a defined minimal medium.
Here is a receipe that worked quite well in our hands:
http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Expression_of_SeMet_labeled_proteins
HTH
Guenter
Dear Crystallographers,
I have been using an E coli strain called Tuner (DE3) pLacI, which is
not auxotrophic for methionine, nor is there an auxotrophic analogue
available. It may be that my protein can be expressed in other
strains, but this one works well right now, and it is usually better
to stay with what works well. Has anybody tried growing semet protein
in non-auxotrophic strains, or are there tricks to using
non-auxotrophs for semet protein?
Thanks,
Jacob
*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: [EMAIL PROTECTED]
*******************************************
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***********************************
Priv.Doz.Dr. Guenter Fritz
Fachbereich Biologie
Sektion Naturwissenschaften
Universitaet Konstanz
http://www.biologie.uni-konstanz.de/fritz
Universitaetsstrasse 10
Postfach M665
D-78457 Konstanz
e-mail: [EMAIL PROTECTED]
Tel. Office: +49-(0)7531 88 3205
Tel. Lab : +49-(0)7531 88 3687
Fax: +49-(0)7531 88 2966
