Hi I also like to try the Hampton additive kit or the easier version; 90% current condition (at 1.1x) and 10% of any screen you can lay your hands on (ie hampton 1 & 2). Sitting verse hanging drops can change crystal size. Also in situ limited proteolysis, crazy but it works see for more info and references. Nature Methods - 4, 1019 - 1021 (2007) In situ proteolysis for protein crystallization and structure determination
Mark On Tue, 14 Oct 2008, shivesh kumar wrote: > Dear all, > I have crystallized a protein in MPD which is thin and growing in one > direction only. I have tried ethonal, DMSO, Proline, Glucose, Urea, Sucrose, > Detergent kit from Hampton and lot more alongwith temperature > variation. Need suggestions regarding improvement of xtal quality, > THICKNESS. > Thanx in advance. > Shivesh >
