2008/9/25 amit sharma <[EMAIL PROTECTED]> > > Dear CCP4bbers, > I have a protein molecule(~9.0 kDa) that crystallized in the presence of > 0.15M KBr and HEPES pH 7.0. Since there is no homologuous structure present, > I intend to perform heavy metal derivatization. I read some literature which > suggested that I could carry out quick soak with 0.5M Sodium iodide. I was > wondering if I could soak my crystals with a similar concentration of NaBr > and collect some low resolution data at the in-house source? In addition > should I try to also introduce other heavy metals? it might be worth > mentioning that my protein carries no methionine/cys residues. > I have no prior experience in doing this. So, it would be of great help if I > could be directed towards literature/protocols pertaining to this. Any > advice/suggestions would be greatly appreciated. > > Thanks in advance > -- > Amit Sharma
One solution can be soaking or co-crystallization with lanthanide complexes. More information available in these publications (PubMed): PMID: 15272192 PMID: 14573945 PMID: 12499547 PMID: 18350532
