Hi Amit, http://www.doe-mbi.ucla.edu/~sawaya/tutorials/Phasing/references.html
And the (IMHO) seminal heavy atom derivative reference: Petsko, G.A., "Perparation of Isomorphous Heavy-Atom Derivatives" Methods in Enzymology, Volume 114, , pages 147-157. should give you all the info you need. The 0.15M KBr might give you a usable anomalous signal, but the only way you'll be able to determine that is to go ahead and do it! the f" of Br @ CuKa is ~1.2e-, with careful data collection, you might get lucky. Cheers, David 2008/9/25 amit sharma <[EMAIL PROTECTED]>: > > Dear CCP4bbers, > I have a protein molecule(~9.0 kDa) that crystallized in the presence of > 0.15M KBr and HEPES pH 7.0. Since there is no homologuous structure present, > I intend to perform heavy metal derivatization. I read some literature which > suggested that I could carry out quick soak with 0.5M Sodium iodide. I was > wondering if I could soak my crystals with a similar concentration of NaBr > and collect some low resolution data at the in-house source? In addition > should I try to also introduce other heavy metals? it might be worth > mentioning that my protein carries no methionine/cys residues. > I have no prior experience in doing this. So, it would be of great help if > I could be directed towards literature/protocols pertaining to this. Any > advice/suggestions would be greatly appreciated. > > Thanks in advance > -- > Amit Sharma -- ============================ David C. Briggs PhD Father & Crystallographer http://drdavidcbriggs.googlepages.com/home AIM ID: dbassophile ============================
