Thanks Guenter and Andreas, Yea, I have taken a look for the Mono P before, I thought the material they used in Mono P is basically the same as in Mono Q and I found the book"Protein Purification Protocols: Second Edition" by Paul Cutler mentioned that phosphate buffer can also generate a continuous gradient....that's why I reckon we can also perform pH gradient in Mono Q. But I am worrying if it is a good idea to go for it, as both mono Q or mono P are quite expensive.
Matt 2008/6/24 Andreas Förster <[EMAIL PROTECTED]>: > Hey Matt, > > it seems to me that what you're asking for is chromatofocusing. See the > official GE documentation: > > http://www6.gelifesciences.com/aptrix/upp00919.nsf/Content/WD:Chromatofocusin(260949098-R350)<http://www6.gelifesciences.com/aptrix/upp00919.nsf/Content/WD:Chromatofocusin%28260949098-R350%29> > > The proprietary buffers are a bit expensive, but as you found out, they're > a bit complicated to make. I don't know if you'd ruin your Mono Q with a pH > gradient. If in doubt, buy one of the dedicated Mono P column. > > Hope that helps. > > > Andreas > > > Matthew Chu wrote: > >> Dear All, >> >> Sorry for off-topic question. Does anyone have any experience in purifying >> protein using pH gradient in Mono Q column? >> >> I have been googling for a whole day, only one paper was found to mention >> performing pH gradient in Mono Q, but in a mixture of amine buffering >> species, which is a bit too complicated (J. Chromatogr. A 1164 (2007) 181 - >> 188. Can Tris-Cl/Tris-base or phosphate buffer give a linear pH gradient >> from pH 8.0 to 4.0? Is it usual to perform pH gradient in Mono Q as I don't >> want to ruin my Mono Q column... >> >> Any suggestions are welcome. Thanks in advance! >> >> Kind regards, >> Matt >> >> >> ---------------------------------------------------------------------------- >> Matthew LH Chu >> PhD Student >> School of Pharmacy and Pharmaceutical Sciences >> University of Manchester >> >> ---------------------------------------------------------------------------- >> > -- ---------------------------------------------------------------------------- Matthew LH Chu PhD Student School of Pharmacy and Pharmaceutical Sciences University of Manchester ----------------------------------------------------------------------------
