Hey Matt,
it seems to me that what you're asking for is chromatofocusing. See the
official GE documentation:
http://www6.gelifesciences.com/aptrix/upp00919.nsf/Content/WD:Chromatofocusin(260949098-R350)
The proprietary buffers are a bit expensive, but as you found out,
they're a bit complicated to make. I don't know if you'd ruin your Mono
Q with a pH gradient. If in doubt, buy one of the dedicated Mono P column.
Hope that helps.
Andreas
Matthew Chu wrote:
Dear All,
Sorry for off-topic question. Does anyone have any experience in
purifying protein using pH gradient in Mono Q column?
I have been googling for a whole day, only one paper was found to
mention performing pH gradient in Mono Q, but in a mixture of amine
buffering species, which is a bit too complicated (J. Chromatogr. A 1164
(2007) 181 - 188. Can Tris-Cl/Tris-base or phosphate buffer give a
linear pH gradient from pH 8.0 to 4.0? Is it usual to perform pH
gradient in Mono Q as I don't want to ruin my Mono Q column...
Any suggestions are welcome. Thanks in advance!
Kind regards,
Matt
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Matthew LH Chu
PhD Student
School of Pharmacy and Pharmaceutical Sciences
University of Manchester
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