The primary reason they don't have much on the Sephadex resins is
that the are quite old-fashioned (and also relatively cheaper than
most of their high-end resins). However, old Pharmacia/LKB had some
really great manuals on chromatography that are still quite useful
for new biochemists. As they have morphed into GE Healthcare, search
on their website for 18-1022-18 (Gel Filtration: Principle and
Methods) or 18-1114-21 (Ion Exchange Chromatography) and all you want
to know about Sephedex resins will be in these free downloadable
manuals. There are lots of other useful manuals in their collection.
On caveat about Sephedex resins, anything above G-75 may compress in
an FPLC system. So don't try to do gel filtration with Sephedex
G-150. Ion exchange chromatography with G-25 or G50 based resins
should be OK.
Cheers,
Michael
****************************************************************
R. Michael Garavito, Ph.D.
Professor of Biochemistry & Molecular Biology
513 Biochemistry Bldg.
Michigan State University
East Lansing, MI 48824-1319
Office: (517) 355-9724 Lab: (517) 353-9125
FAX: (517) 353-9334 Email: [EMAIL PROTECTED]
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On Jun 23, 2008, at 7:11 AM, Exec wrote:
does anyone have protocols / method of sephadex resin. about
swelling the
resin use of buffers, elution of sample from the resin. i have
referred to
the GE healthcare website, it gives details of other resins well, but
sephadex resin not much detail is given.
kindly guide
