Any basic chromatography (for proteins) text will have those. It's generally not hard - if you have dry sephadex just resuspend it in 10-20% alcohol, stir well (but gently!) and let sit overnight, then pack the column, wash, and go.
Artem > does anyone have protocols / method of sephadex resin. about swelling the > resin use of buffers, elution of sample from the resin. i have referred to > the GE healthcare website, it gives details of other resins well, but > sephadex resin not much detail is given. > > kindly guide >
