Mercurochrome (mebromin) is both a derivative AND a stain (tested, true). It's just like having a pie and eating it at the same time.
Artem -----Original Message----- From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Richard Gillilan Sent: Sunday, June 15, 2008 9:36 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] alternatives to IZIT We've tested eight different dyes carefully on three different proteins and eventually planned to test a much larger number (I''ve assembled a large collection of dyes of every color and type). So far everything has worked! (rhodamine, bromophenol blue, and fluorescein are my favorites because they are fluorophores). You could probably pick almost anything out of HJ Conn's book "Biological Stains." I believe there was some trouble with mixes containing high PEG conc, but I don't recall the details at the moment. You might even consider using a dye with high bromine content (like bromophenol blue) to phase the structure, but that would require much higher concentrations than are typically necessary in ISIT-like experiments. We did see some evidence of change in crystal morphology when the proteins where grown with the dyes as opposed to soaked. Not sure how significant that effect is. Obviously a dye could potentially induce conformational or oligomeric changes in some cases. Richard Gillilan MacCHESS Ithaca, NY On Jun 14, 2008, at 4:51 PM, Mark Del Campo wrote: > Before I place an order for some Izit, are there some other dyes I > can use to check if I've got a protein > crystal? > > Thanks, > > Mark
