Hi,John,
I faced the similar situation before even the resolution about 2.4. At
that region the residues are all hydrophobic ones and should be very
flexible . Arp/warp and phenix do not work well and resolve does not
improve the density either. At the end I just try to build the model
as I can . That is all for me and hope someone can give us some good
suggestions .
Good luck!
leo
john kryst wrote:
Hi all !!
I am working with two data sets of same protein (130a/a) with
resolutions 2.8 and 3.2 A. In both the cases the density for 100-130
aa is not very clear.. it forms couple of helices.. i can see a long
tube going but it is feature less.. It is a MR solution.. i have tried
TLS refinement with different TLS groups.. but no luck... i have also
tried DM but not much improvement... Data looks clean without any
twinning... R and Rfee are around 22.8 and 28.5 for both the
structures... Any suggestions??
regards
John
