Weikai,
You might look up these references:
A. Lustig et al. "Density determination by analytical
ultracentrifugation in a rapid dynamical gradient: application to
lipid and detergent aggregates containing proteins." Biochim.
Biophys. Acta 1115 (1991) 85-95.
Ariel Lustig et al. "Molecular weight determination of membrane
proteins by sedimentation equilibrium at the sucrose or Nycodenz-
adjusted density of the hydrated detergent micelle." Biochim.
Biophys. Acta 1464 (2000) 199-206
Ariel Lustig, a master of the old model E and the new Beckman
instrument, did a lot of work for Jurg Rosenbusch just on this
subject. One other aspect that needs to be addressed is the
compressibility of the detergent micelles. This can vary from
detergent to detergent. You can find much of what you want by
delving into the surfactant/colloidal science literature.
Also look up the work of Marc le Maire, who has studied detergent-
protein interactions for years. If you can, get a copy of Michael
Grabo's thesis (Solubilization and Reconstitution of Membrane
Proteins, Ph. D. Thesis 1982. University of Basel, Switzerland);
there are many useful nuggets in this work.
Michael
****************************************************************
R. Michael Garavito, Ph.D.
Professor of Biochemistry & Molecular Biology
513 Biochemistry Bldg.
Michigan State University
East Lansing, MI 48824-1319
Office: (517) 355-9724 Lab: (517) 353-9125
FAX: (517) 353-9334 Email: [EMAIL PROTECTED]
****************************************************************
On Jun 5, 2008, at 5:57 PM, [EMAIL PROTECTED] wrote:
Hi:
Just a related question. To measure MW of membrane proteins, the
density
of detergents needs to be matched by D2O/H2O and should be within
1.0 to
1.1. Anyone knows some literature that gives a list of detergents
having
suitable density? Unfornatatedly, the common ones like Foscholine
12(DPC)
and C12E9 do not work with my protein.
Regards,
Weikai
Hi Mike,
try the National Analytical Ultracentrifugation Facility in
Connecticut.
http://www.biotech.uconn.edu/auf/
Cheers, Petra
Dear colleagues,
does anyone know of a faciltiy for analytical ultracentrifugation?
Thanks.
Mike Colaneri
--
Petra Verdino, PhD
Research Associate
The Scripps Research Institute, BCC206
10550 North Torrey Pines Road
CA 92037 La Jolla, USA
tel:1-858-784-2945
fax:1-858-784-2980
http://www.scripps.edu/~verdino/homepage.htm
