You might have to test if it is salt/detergent or protein crystal first. If it is really protein crystal, never give up and for needle crystal, generally it is difficult to optimze, however in most cases, the diffraction is well enough. If it is protein crystal, as some researcher has mentioned, first try Hampton additive screening, if you are lucky, you might get an additive condition which might improve the thickness of needle or plate to some thickness, if additive screening does not improve much to your protein crystal, you have to try seeding.
HTH Donghui On 4/16/08, Ngo Duc Tri <[EMAIL PROTECTED]> wrote: > > Dear ccp4 users, > I crystallized a membrane protein and got some conditions showing the > small needle crystals. All condition contained Mg2+ and high buffer pH. > I learn that it is difficult to optimize the needle crystal. However I > would like to ask your experience how to optimize in this case. > Here is the attached picture of my crystal. Thank you very much for your > advices! > > My best regards, > TriNgo > PhD Student - Sungkyunkwan University, Korea > >
