We also have this machine (Cartesian Honeybee X8 ) and can verify that
dispensing 3 drops per condition increases the overall build time about
30 seconds.
We have also recently acquired a Cartesian Honeybee 963 which will do
the whole experiment setup, including dispensing precipitant into the
well and onto the shelf, in 6 minutes for 2 protein sites (including all
washes). A 3 site experiment would take 30 seconds longer. The actual
time from first drop dispense to last is about 1 minute.
Stephen
SSPF
University of St Andrews
We use Cartesian Honeybee X8 machines (8 tips). They take about 10 minutes to set a 96-drop plate including the washes of the tips. 3 or 4 drops per condition wouldnt take much longer. Optimisation and additive/detergent screens take a little less time.
The plates are pipetted under a close-fitting cover to (virtually) eliminate
evaporation, which IMO is better than a humidity chamber. Consumable costs
extend to isopropanol and water, with the occasional replacement valve or tip.
Since people here also tend to turn up at beer o'clock on a Friday evening
(must be an Oxford thing...) we have two machines (and another one imminent) to
increase throughput.
HTH
Tom
** Tom Walter B.Sc. M.Res. **
** Oxford Protein Production Facility Tel: +44 (0)1865 287747 **
** Wellcome Trust Centre for Human Genetics Fax: +44 (0)1865 287547 **
** Roosevelt Drive [EMAIL PROTECTED] **
** Headington, Oxford OX3 7BN http://www.oppf.ox.ac.uk **
---- Original message ----
Date: Mon, 14 Apr 2008 17:10:26 -0400
From: JOE CRYSTAL <[EMAIL PROTECTED]>
Subject: Re: [ccp4bb] Fwd: [ccp4bb] crystallisation robot
To: [email protected]
Hi,
Does anyone have information about how long it takes to set up
a 96-well tray for the crystallization robots available?
Besides cost per tray and maintenance cost, another important
feature we consider is the time for setting up a 96-well
tray. It is an important factor since we are talking about
sub-microliter drops.
Best,
Joe
On Fri, Jan 18, 2008 at 12:28 PM, Lisa A Nagy
<[EMAIL PROTECTED]> wrote:
Al's Oil on the plates:
What a nightmare!!!!!!!
The oil creeps up the plate and over the sides. It dissolves
adhesives.
It makes me say bad words in multiple languages.
Bigger drops + no oil = fewer bad words.
Lisa
--
Lisa A. Nagy, Ph.D.
University of Alabama-Birmingham
[EMAIL PROTECTED]
-----Original Message-----
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On
Behalf Of
Patrick Shaw Stewart
Sent: Friday, January 18, 2008 2:20 AM
To: [email protected]
Subject: [ccp4bb] Fwd: [ccp4bb] crystallisation robot
One thing that people often overlook is that quite a lot of
protein
can be lost by denaturation on the surface of the drop.
This is more
significant for smaller drops. Two suggestions: (1)
increase the
proportion of protein in the - technical term - teeny drop
to say two
thirds and (2) cover the drops with oil eg Al's oils
(silicone/paraffin). You still get vapor diffusion though
the oil ,
and you'd like to slow up equilibration. of course (2)
slows up the
robotics a little, but both should be trivial to set up..
