I'm not entirely sure this paper will answer your question, as I can't access the full article at home, but I seem to remember it may contain what you seek. It's certainly worth giving it another airing...
http://scripts.iucr.org/cgi-bin/paper?S0907444905002726 Acta Cryst. (2005). D61, 490-493 [ doi:10.1107/S0907444905002726 ] Expanding screening space through the use of alternative reservoirs in vapor-diffusion experiments J. Newman HTH Dave On 07/04/2008, Kay Diederichs <[EMAIL PROTECTED]> wrote: > Dear all, > > a protein which we work on is available in low quantity. The only > crystallization screen we set up is completely clear, no precipitate, > nothing. > > Now we would like to modify the reservoirs of this screen, by adding LiCl > or Ammoniumsulfate or ... , with the goal of reducing the vapour pressure, > to at least get the protein concentration in the drop into the range where > "something happens". > > Does anyone have advice as to which salt we should add (to the reservoir > only)? AmSO4 is only soluble to 4M, LiCl goes to 10M. But vapour pressure > reduction is not the same as molarity. > > thanks for any insight, > > Kay > -- > Kay Diederichs > http://strucbio.biologie.uni-konstanz.de > email: [EMAIL PROTECTED] Tel +49 7531 88 4049 Fax 3183 > Fachbereich Biologie, Universität Konstanz, Box M647, D-78457 Konstanz > > -- ============================ David C. Briggs PhD Father & Crystallographer http://www.dbriggs.talktalk.net AIM ID: dbassophile ============================
