Dear Yuhua I think that you may have failed to specify a solvent content fraction before attempting to run DM - please try rerunning the job and checking the "Required Parameters" folder about halfway down the window. If the box next to the text "Fraction solvent content" is empty and coloured yellow then this is likely to be the problem.
The solvent fraction should be between 0.0 (all protein, no solvent) and 1.0 (all solvent, no protein). Please check the DM documentation for more information. Hope that this helps, best wishes Peter yuhua wrote: > Dear Crystallographers, > > I failed to run DM in ccp4 package recently and got the errors as below: > > <a href="http://www.yorvic.york.ac.uk/~cowtan/dm/refs.html";>dm > reference:</a> > <blockquote> > K. Cowtan (1994), > dm: An automated procedure for phase improvement by density modification. > Joint CCP4 and ESF-EACBM Newsletter on Protein Crystallography, 31, > p34-38. > </blockquote><p> > > > <a name="tocdm"><h2>Contents</h2></a> > <ul> > <li><a href="#commanddm">Command input</a> > <li><a href="#commentsdm">Comments</a> > <li><a href="#mtzindm">MTZ input</a> > <li><a href="#datachkdm">Data Checking</a> > <li><a href="#datascldm">Data Scaling</a> > <li><a href="#solmskdm">Solvent Mask</a> > <li><a href="#cyc0001dm">First Cycle</a> > <li><a href="#dataoutdm">Output</a> > </ul> > > <a name="commanddm"><h2>Command Input</h2></a> > <pre> > Data line--- <a href="/usr/local/ccp4-6.0.2/docs/dm.html#mode">MODE > </a> SOLV > Data line--- <a > href="/usr/local/ccp4-6.0.2/docs/dm.html#combine">COMBINE </a> PERT > Data line--- <a > href="/usr/local/ccp4-6.0.2/docs/dm.html#scheme">SCHEME </a> ALL > Data line--- <a > href="/usr/local/ccp4-6.0.2/docs/dm.html#ncycle">NCYCLE </a> AUTO > Data line--- <a href="/usr/local/ccp4-6.0.2/docs/dm.html#solc">SOLCONT > </a> > > *** Warning > Real sub-argument expected > > Data line--- <a > href="/usr/local/ccp4-6.0.2/docs/dm.html#ncsmask">NCSMASK </a> > Data line--- <a href="/usr/local/ccp4-6.0.2/docs/dm.html#labin">LABIN > </a> FP=F_p2 SIGFP=SIGF_p2 PHIO=PHIC FOMO=FOM > Data line--- <a > href="/usr/local/ccp4-6.0.2/docs/dm.html#labout">LABOUT </a> FDM=FDM > PHIDM=PHIDM FOMDM=FOMDM > dm: Input error (see above) > Times: User: 0.0s System: 0.0s Elapsed: 0:00 > </pre> > </html> > *************************************************************************** > * Information from CCP4Interface script > *************************************************************************** > The program run with command: dm HKLIN > "/Users/Research/CNS/newtls_refmac4.mtz" HKLOUT > "/Users/Research/CNS/newtls_dm1.mtz" SOLOUT > "/Users/Research/CNS/newtls_dm1.msk" > has failed with error message > dm: Input error (see above) > *************************************************************************** > > > #CCP4I TERMINATION STATUS 0 " dm: Input error (see above)" > #CCP4I TERMINATION TIME 04 Mar 2008 22:15:09 > #CCP4I MESSAGE Task failed > > > I can not fix it. Can anyone give me some help? > Thanks very much! > -- ___________________________________________________ Peter J Briggs, [EMAIL PROTECTED] Tel: +44 1925 603826 CCP4, [EMAIL PROTECTED] Fax: +44 1925 603825 http://www.ccp4.ac.uk/ Daresbury Laboratory, Daresbury, Warrington WA4 4AD
