Hi Sun,
I suggest checking that the b-factors for those residues aren't
unexpectedly high compared to those in the flankinh regions.
Cheers,
James


On 1/23/08, Sun Tang <[EMAIL PROTECTED]> wrote:
> Hello Everyone,
>
>   When I refined a structure, I found strong difference density Fo-Fc at 3
> sigma contour for the for five residues which already have occupancy of 1.
> The density is continuous and so strong as if I did not put the residues
> there. Why was that? Can I put greater than 1 occupancy for those residues?
>
>   Thank you very much for your opinions and suggestions!
>
>   Best wishes,
>
>   Sun Tang
>
>
> ---------------------------------
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Dr. James Irving
NH&MRC C.J. Martin Fellow
School of Biomedical Sciences
Building 13D
Monash University
Wellington Road
Melbourne 3800
Australia

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