Hi Sun, I suggest checking that the b-factors for those residues aren't unexpectedly high compared to those in the flankinh regions. Cheers, James
On 1/23/08, Sun Tang <[EMAIL PROTECTED]> wrote: > Hello Everyone, > > When I refined a structure, I found strong difference density Fo-Fc at 3 > sigma contour for the for five residues which already have occupancy of 1. > The density is continuous and so strong as if I did not put the residues > there. Why was that? Can I put greater than 1 occupancy for those residues? > > Thank you very much for your opinions and suggestions! > > Best wishes, > > Sun Tang > > > --------------------------------- > Never miss a thing. Make Yahoo your homepage. -- Sent from Gmail for mobile | mobile.google.com Dr. James Irving NH&MRC C.J. Martin Fellow School of Biomedical Sciences Building 13D Monash University Wellington Road Melbourne 3800 Australia