Vaheh,
If I'm optimising in 24 well trays after a hit in 96 I generally use
sitting drop trays (cryschem) as a first step and try different ratios
of protein:mother liquor first rather than changing the protein
concentration. I know that sometimes the crystals stick to the plastic
but I use the hypodermic trick mentioned on the BB a few days ago.
On the subject of drop size - I was at the RAMC meeting where this was
discussed and several attendees had looked at the issue, 300+300nl or
400+400nl were mentioned as more optimal drop sizes for obtaining
crystal hits during screening.See summary on:
http://www.hamptonresearch.com/stuff/RAMC/RAMC2007Notes.aspx
At York we use 150+150nl routinely for screening using the mosquito.
I've moved up to 200+200 since the conference!
Shirley Roberts
ps I think I was responsible for the z to s change in the title....
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