Have you tried TLS and NCS? Maybe these can lower the R free a little.
But ,the quality of your model depends on the quality of your data and
completeness of your model etc.
Yongchao Li wrote:
I have a large molecule to refine.There are about three thousand
residues in one asymmetric unit. Except for the lack of electron
densities in several places, where the loops have been deleted, the
rest parts fits well with density. But the R free and R work are all
beyond 0.30 (R free = 0.35, R work = 0.30).
I don't know how to reduce them. Can anyone give me some ideas?
Thank you!
---------------------------------
Looking for last minute shopping deals? Find them fast with Yahoo!
Search.
Size alone is not a problem.. think of viruses
But it is easy to make errors of course, and more time consuming to
check in such a long chain
what is the resolution though..
