Re: [ccp4bb] Is phophorylation possible in E. coli expression system?

[mesters]

Hi, Elongation factor Tu was one of the first E. coli proteins that was identified as being phosphorylated given certain conditions (early 90s)..... What I find interesting is the fact that EF-Tu becomes hyperphosphorylated if the cells grow at 20 degrees. Maybe this is also true for other proteins that are abundantly expressed.... Did you reduce the _expression_ temperature from 37 to 20 degrees? J. El-Fahmawi B, Owttrim GW. Related Articles, Links Cold-stress-altered phosphorylation of EF-Tu in the cyanobacterium Anabaena sp. strain PCC 7120. Can J Microbiol. 2007 May;53(5):551-8. Lippmann C, Lindschau C, Vijgenboom E, Schroder W, Bosch L, Erdmann VA. Related Articles, Links Prokaryotic elongation factor Tu is phosphorylated in vivo. J Biol Chem. 1993 Jan 5;268(1):601-7. Alexander C, Bilgin N, Lindschau C, Mesters JR, Kraal B, Hilgenfeld R, Erdmann VA, Lippmann C. Related Articles, Links Phosphorylation of elongation factor Tu prevents ternary complex formation. J Biol Chem. 1995 Jun 16;270(24):14541-7. -- Jeroen Raymundus Mesters, Ph.D. Institut fuer Biochemie, Universitaet zu Luebeck Zentrum fuer Medizinische Struktur und Zellbiologie Ratzeburger Allee 160, D-23538 Luebeck Tel: +49-451-5004070, Fax: +49-451-5004068 E-mail: [EMAIL PROTECTED] Http://www.biochem.uni-luebeck.de Http://www.iobcr.org Http://www.opticryst.org -- If you can look into the seeds of time and say which grain will grow and which will not - speak then to me (Macbeth) --