Dear Jacob
The main reason why different programs produce different resutls is
that methods used in these programs are different.
For example refinement programs use different methods for scaling.
Some include bulk solvent a Babinet, some use
mask bulk slvent and some use anisotropic scaling others do not use.
Even for each parameterisation there are variations.
Fortunately for most of the cases the results are mathematically
equivalent and maps do not change too much.
Regards
Garib
On 7 Sep 2007, at 16:27, Jacob Keller wrote:
Dear list,
I have for some time now wondered why different programs output
different statistics. A low FOM
from program A might be much better than a high FOM from program B,
and so on. I wonder why, then,
considering that statistical measures are precisely, mathematically
defined, how is there any
discrepancy? I have also wondered whether people might prefer
certain programs because they are
statistically flattering. I think in my experience I have seen even
statistics like Rfree to be
different from different programs, I think even without any
refinement--so should one use that
program last, right before composing "Table I" for publication?
That seems suspicious...
Jacob Keller
==============Original message text===============
On Fri, 07 Sep 2007 3:39:27 am CDT Andreas Kohl wrote:
Dear all,
we have currently two open postdoctoral positions in our
department. We
would very much appreciate it if you could bring this announcement to
the attention of suitable candidates.
All inquiries and applications should be send to:
Prof. Pär Nordlund ([EMAIL PROTECTED]) or Dr. Said Eshaghi
([EMAIL PROTECTED])
Andreas
######################################################################
##############
Postdoctoral positions available at Karolinska Institutet, Sweden
Two postdoctoral positions are available at the division of Biophysics
in the department of Medical Biochemistry and Biophysics, at
Karolinska
Intitutet in Stockholm. The division is headed by Professor Pär
Nordlund
and is focused on functional characterization of proteins, primarily
using X-ray crystallography. In addition, new technologies are being
developed and improved as tools to enable high-throughput approaches
within protein production. The group has a strong record in structure
determination of soluble and membrane proteins. The laboratory is
well-equipped with state-of-the-art instruments for protein production
and crystallization.
Currently, two postdoctoral positions are available in the membrane
protein group, working with medically important proteins from
different
families, such as solute transporters, ion channels and enzymes:
1. Membrane protein chemistry/structural biology. The applicant should
have strong background in recombinant membrane protein production
in E.
coli system, preferably with some experience in protein
crystallization.
The main topic is to develop new and improved methods for purification
and crystallization of integral membrane proteins. Knowledge about
X-ray
crystallography is ideal but not a requirement. The successful
candidate
will be part of a dynamic team that is working on production,
crystallization and structure determination of membrane proteins. The
applicant must therefore have the ability to work as a team-player as
well as independently. The position is initially announced for two
years.
2. Membrane protein X-ray crystallography. The applicant should have a
strong background in X-ray crystallography with a good track
record. The
main tasks are crystallization screening, crystal optimization, data
collection and structure determination of integral membrane proteins.
Previous experience with membrane proteins is ideal but not a
requirement. The successful candidate will be part of a team that is
working on production and crystallization of membrane proteins. The
applicant must therefore have the ability to work as a team-player as
well as independently. The position is initially announced for two
years.
Applicants should send a full CV, including a publication list,
together
with the name and contact details of three references to:
Prof. Pär Nordlund ([EMAIL PROTECTED]) or Dr. Said Eshaghi
([EMAIL PROTECTED])
----------------------------------------------------------------------
---------------------------------------------
Prof. Pär Nordlund
[EMAIL PROTECTED]
Institutionen för Medicinsk Biokemi och Biofysik (MBB)
Karolinska Institutet, 171 77 Stockholm
Biofysik
===========End of original message text===========
***********************************
Jacob Keller
Northwestern University
6541 N. Francisco #3
Chicago IL 60645
(847)491-2438
[EMAIL PROTECTED]
***********************************
