Hello All, I have two questions about refinement with Refmac5.
1. I have a protein structure that is C-terminally amidated. What is the best way to handle this? I was trying to use the sketcher to create my own library definition for the modifed amino acid, but then I had issues with the modified amino acid not being covalently linked to the rest of the protein. I'm guessing there is a better way, such as creating a definition for just the additional NH2 group and telling refmac how to covalently link it to the last amino acid, but I'm not sure how to do this. 2. I have a structure with maltose in it. The refmac library definition for maltose has atoms "O1" and "O1," mislabeled as Carbons. How can I correct this? I know I can change it with the sketcher, but then it tells me the name MAL is already taken and I have to use another. I'd prefer to leave it as the standard MAL name. Thanks for any help. I apologize if these issues have been covered before. Augie Augen Pioszak, Ph.D. Postdoctoral Fellow Laboratory of Structural Sciences Van Andel Research Institute 333 Bostwick Ave. N.E. Grand Rapids, MI 49503 phone: (616) 234-5399 email: [EMAIL PROTECTED] This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient(s) please contact the sender by reply email and destroy all copies of the original message. Thank you.
