Hi all, I have crystals of the apo enzyme growing from 1.7M ammonium sulfate condition. After soaking with 10mM ligand (substrate analog) which has a phosphate group, the ligand did not enter the active site of the enzyme because of the competition of sulfate ion and phosphate group. So I am wondering if anyone knows how to get rid of ammonium sulfate from crystal before soaking it with the ligand solution? Thanks a lot.
Simon
