Hi, We use additives extensively. Pretty much all available kits, as well as some of our own design. My favorite is Nextal (Qiagen) kit named the Salt Optimizer.
Artem > Hello everyone, > > Does anyone have suggestions or comments on the various additive > screens that are available? > > I was looking at the kit from Hampton (HR2-138 Additive Screen HT) in > 96-well format. It appears that it should be straight forward to > array out our current protein/mother liquor condition into a 96-plate > and then add in the additives from the deep-well block provided by > the company. The additives come 10x concentrated in a 96-well deep- > well block for dilution into the arrayed crystallization condition. > > Does anyone do this routinely for the crystals they grow? It would > be interesting to know how often people find a systematic approach > using such a kit actually improves the quality/size etc. of their > crystals. > > Cheers, > > Brian > > ------------------------------------------------------- > Brian L. Mark, MSc, PhD > Department of Microbiology > Room 418, Buller Building > University of Manitoba > Winnipeg, Manitoba > CANADA R3T 2N2 > > Phone (204) 480-1430 > Fax (204) 474-7603 > Web: http://www.umanitoba.ca/science/microbiology/staff/mark/
