Dear All, following the thread on 'protease cleavage sites' from early March, I would like to draw your attention to the paper below, describing the directed evolution of a TEV protease with enhanced solubility for production in E coli. I think it might be useful for many of us. 1: van den Berg S, Lofdahl PA, Hard T, Berglund H. Improved solubility of TEV protease by directed evolution. J Biotechnol. 2006 Feb 10;121(3):291-8. Epub 2005 Sep 15.
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