Hello All,
We are trying to crystallize a membrane protein but cannot get the xtals to
grow bigger. Presently we have only thin needles (diffracting to about 8A).
Thus far we have tried to change protein concentration, LDAO concentration, PEG
screen as well as temperature. Have tried macro and micro seeding as well.
A typical setup looks like: 10% PEG 6000, 0.1M Citrate 5.5, 0.1M Li2SO4 and 5%
MPD with about 12mgs/ml of protein which has about 0.2% LDAO
Any suggestions or improvements that we can try would be appreciated. This
construct of the protein is not in the membrane but membrane associated.
Thanks
Balaji
