It depends on scale. With some synthetic DNA companies if you order many at once, you can get a huge discout, down to maybe 35-45 cents per base.
Alternatively, if you have free labor (students) then you can multiparallel your PCR, but $100 per clone is going to be hard to beat given the fact that you still have to buy primers, use PCR reagents and cleanup/cloning kits, etc. etc. and at the end also would want to sequence the products. How many genes are you trying to get? Artem > Sorry a little offtopic... > > We'r trying to clones a number of putative human proteins for > crystallization. Besides IMAGE clones from OpenBiosystem, is there any > the cheaper way of obtaining human cDNA clones? OpenBiosystem > is okish, ~$100Cdn for each clone, but does get abit expensive when you > start getting alot. >
