Hi Jenny (and others),
The buffer systems used in the PACT screen are described in the
following paper by Janet Newman.
/Acta Cryst./ (2004). D*60*, 610-612 [ doi:10.1107/S0907444903029640
<http://dx.doi.org/10.1107/S0907444903029640> ]
Novel buffer systems for macromolecular crystallization
J. Newman
<http://scripts.iucr.org/cgi-bin/citedin?search_on=name&author_name=Newman,%20J.>
I am not aware of any commercial source of these buffer systems. But the
paper describes the recipes to make them, which is quite easy.
HTH
Jose
On 30/01/2007 5:19 Jenny wrote:
Hi,
Sorry to bother you all.I'm going to try to reproduce some results
from the initial screening from PACT kit.The conditions are something
like 0.1M PCB buffer, 25% w/v PEG 1500 and 0.1 MMT buffer, 25%w/v PEG
1500. I was just wondering is there any easy way to get these
buffers?Do you happen to know where to order?Or I have to order the
component formula separately and then make buffers by myself?Thanks.
Jenny
--
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Jose M de Pereda, PhD
Centro de Investigación del Cáncer
Spanish National Research Council - U. Salamanca
Campus Unamuno s/n
E-37007 Salamanca, Spain
Phone: +34-923-294819
Fax: +34-923-294795
http://xtal.cicancer.org/
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