You can try DMI (1,3-dimethyl-2-imidazolidinone) as an alternative to
DMSO. Also, sometimes you get lucky, and the small molecule is more
soluble when high concentrations of glycerol are present, so you can
do the soak in a cryo buffer...
On Jan 22, 2007, at 3:40 PM, Green, Todd wrote:
Hello All,
I am trying to soak some crystals with a small molecule that is
quite hydrophobic. I am having trouble with solubilty of the small
molecule. It will dissolve up to about 1 mM in 100 % DMSO, but
precipitates at concentrations of less than 15 micromolar when the
DMSO concentration is below 20 percent in my crystal growth
solutions(which are peg 4k, low pH, low salt). Can anyone suggest
solvents other than DMSO which might help dissolve the inhibitor
and might be somewhat friendly to my crystals.
Thanks in advance-
Todd Green
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