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But then I guess it is not a 'full 6D search' anymore (but a lot more
dimensions), because you want to position all the proteins, right?
Serge.
Le 23 janv. 07 à 04:14, Bart Hazes a écrit :
Hi Filip,
You're right and the same applies if the MR is difficult because of
differing relative domain orientations in otherwise closely related
to proteins. As mentioned, my remark was aimed at distantly related
search models.
Bart
Filip Van Petegem wrote:
But that isn't necessarily the case if the search is hard because
your
search models individually constitute only a small part of the
asymmetric unit. Say that 80% of the AU consists of multiple
different proteins with known structure; the phase information would
be very high if you find the solutions.
Filip
On 1/22/07, Bart Hazes <[EMAIL PROTECTED]> wrote:
I'd like to add that the value of a molecular replacement
solution tends
to be inversely correlated with the effort needed to find the
solution.
In other words, the harder you have to work to find the MR
solution the
less informative the phase information you tend to get. When you
have
very high resolution and/or NCS you may still be able to solve the
structure. However, in cases were the search model is only distantly
related to the protein of interest and Phaser can't find the
solution,
the solution may not be worth finding and you're better of
focussing on
getting experimental phases.
Bart
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