Perhaps this will be of some use: We've recently published a phosphatase paper showing two structures - one was a soak, the other - a co-crystal. Same ligand and same protein in both, and virtually the same crystallization conditions. The difference in ligand binding was like night and day.
http://scripts.iucr.org/cgi-bin/paper?sx5059 Artem -P&G -P ... I am running out of symbols ... -----Original Message----- From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of [EMAIL PROTECTED] Sent: Monday, January 22, 2007 6:30 PM To: CCP4BB@JISCMAIL.AC.UK Subject: : misbound ligand examples? A biochemist friend asked for examples of cases were a protein was co-crystallized with or soaked in a ligand that bound in the wrong place - say, because the ligand used wasn't quite the right one or because other important ligands were absent. I'm sure such examples are out there, especially when soaks were done at high concentrations, but I'm having trouble thinking of concrete examples. Help? thanks, Phoebe Rice ---------------------------------------------------------------------------- ----------------------------------------------- Phoebe A. Rice Assoc. Prof., Dept. of Biochemistry & Molecular Biology The University of Chicago phone 773 834 1723 fax 773 702 0439 http://bmb.bsd.uchicago.edu/index.html http://www.nasa.gov/mission_pages/cassini/multimedia/pia06064.html
