I know this is an old post but since I dealt with the same problem today I send 
my solution the same.
In my case the problem was caused by a missing library: libdvdcss.
If you don't have it you probably have to activate the medibuntu repository.
The message given by the program is not clear at all anyway.
Hope this helps someone.

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https://bugs.launchpad.net/bugs/445864

Title:
  Impossible to open a *.iso file

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