Thank you Dr. Mooers,
That worked! I’ve been trying to solve that for months. I’ve been able to get
my protein, linker and actin to fuse.
I have now built a chain of five actin and I’m encountering another barrier.
I’m attempting to link a protein to each actin, but the program recognizes all
Dear all, I have a task to overlay 500+ structures onto a reference
structure "ref", as the body of data is big, I decided to do them in
chunks, 100 at a time (after crashing pymol on my Windows laptop for a few
times attempting it). This is the commend that I used for the first 100:
alignto ref,
Hi Patricia,
You can add unique segment identifiers to each actin.
https://www.biostars.org/p/129381/
You can add these segids by using column selection in
a text editor like Visual Studio Code, which is free.
Install the BioSyntax plugin to get syntax highlighting
of the pdb file.
Best reg
Hi Yong,
Maybe you tried this already, but you might try reducing the use of RAM by
superposing all 500 structures represented as CA traces
('ribbons' in PyMOL) or even show the CA atoms as lines (show lines, name ca
and i. *) and then switch representation after alignment.
Best regards,
B
