Re: [ccp4bb] Insect cell SF9 expression

2013-12-07 Thread nathanielclar5 .
Dhananjay, Hopefully you are using the 'Bac to Bac' system, not the older version 'Bac-N-Blue'. I'd recommend getting a high-titer virus to use as a positive control for infection. Baculovirus is challenging because before you can check for protein expression, you need to do virology experiments.

Re: [ccp4bb] metal chelation

2014-05-19 Thread nathanielclar5 .
Are you treating all your buffers with metal chelating resin? Are you washing all plasticware with EDTA and metal-free buffer prior to use? How are you quantifying your metal content, and what metal ions are contaminating your sample? You might be pulling out the metal ions, but they get right b

Re: [ccp4bb] 3-D Coot Capable Computer Monitor/Glasses for Mac Laptop?

2018-03-02 Thread nathanielclar5 .
You already have stereo built in, just turn on 'cross eyed stereo' and learn to cross your eyes! It's brighter than any 3d monitor, requires no hardware, and no, it won't hurt your eyes (even for hours at a time) Nat On Fri, Mar 2, 2018 at 11:01 AM, Jim Fairman wrote: > Daniel, > > To best

Re: [ccp4bb] 3-D Coot Capable Computer Monitor/Glasses for Mac Laptop?

2018-03-02 Thread nathanielclar5 .
ot;cross eye", I may need to check if some video > instruction online... > > On Fri, Mar 2, 2018 at 11:28 AM, nathanielclar5 . wrote: > You already have stereo built in, just turn on 'cross eyed stereo' and > learn to cross your eyes! It's brighter than any 3d monitor

Re: [ccp4bb] 3-D Coot Capable Computer Monitor/Glasses for Mac Laptop?

2018-03-02 Thread nathanielclar5 .
On Fri, Mar 2, 2018 at 1:41 PM, nathanielclar5 . wrote: > To learn how to cross eyes, just hold you index fingers strait up, ~12-18 > inches from your eyes, and cross your eyes until the two fingers overlap to > form a third finger in the middle. Then practice holding your eyes the