Hi,folks,
I got a brand new Mac Intel. I download the pre-built
mosflm 7.0.1 from Harry's website.But I got the
following error when I try to execute it,
dyld: Library not loaded: /usr/X11R6/lib/libXt.6.dylib
Referenced from:
/Users/mnelson/downloads/./ipmosflm_osx_intel
Reason: image not found
Sorry folks, but I have been very frustrated to get
mosflm and imosflm to work.
I have get around with X11, but I got another problem,
>> CCP4 library signal ccp4_general:Cannot open
environ.def (Success)
raised in ccp4fyp <<
ipmosflm_universal: Cannot open environ.def
ipmosflm_
In my understanding, unbiased electron density map usually refers to the Fo-Fc
map.But I have seen in some papers sentences like that "The initial, unbiased
2FoFc map is contoured at".I was a bit confused since I was told by my
instructor that the 2Fo-Fc was usually biased.
Can anyone clear up
was working to set up an FAD enzymatic assay. I wished to be able to use 450nM
to continuously monitor the progress of the reaction. The substrate I used is
the natural substrate of the enzyme and the protein is recombinant protein and
I assume it's active since I do see changes in TLC plate. B
Dear all,
Thank you for all your kind replies.
Here is a little bit more about the enzyme and how I carry out the assay at the
first place.
My enzyme is a lipid desaturase, originally from plant but overexpressed in
bacteria. FAD serves as a co-factor for this enzyme, in which FAD is reduced t
d then add them to Tris-Chaps buffer (pH ~7, and
about 10mM Chaps). Is there a better way to dissolve them for assay.
Please advise.
michael nelson wrote:
> Dear all,
>
> Thank you for all your kind replies.
>
> Here is a little bit more about the enzyme and how I carry out the
You might be right. I can not rule out the possibility.
By the way, is the PMS/DCPIP system fairly stable in the aerobic condition?
Mike
--- On Thu, 12/4/08, [EMAIL PROTECTED] <[EMAIL PROTECTED]> wrote:
From: [EMAIL PROTECTED] <[EMAIL PROTECTED]>
Subject: Re: [ccp4bb] Offtopic: FAD enzymatic ass
