[ccp4bb] Build the sugars in COOT

2009-12-30 Thread JXQI
Dear All: Sorry for this off-topic subject. This maybe an old topic, but I could not find the previous discussion about it. There are some sugars, i.e.,NAD and NDG that form covalent bonds with ASN in my protein.I tried to load the NAG by "Got Monomer" in COOT, but I couldn't build the co

[ccp4bb] Refinement with simulated annealing

2007-02-27 Thread JXQI
Dear everyone: An off topic question. I first build up a model using ARP/wARP,delete the "DUMP" atoms, add the lost parts directly from the existing model(because of the sequence identity) and then refine it with simulated annealing program(anneal.inp) in CNS.The error is:

[ccp4bb] CNS refinement problem

2008-09-06 Thread jxqi
Dear ccp4bbs, When I was refining a protein structure using the rigid.inp in CNS(Version 1.21), an error occured saying that the asymmetric map unit is incompatible with the symmetry operator.The model I used was first refined by the Rafmac5.I had met the same problem when I tried to add water

[ccp4bb] Phoenix robot

2008-09-09 Thread jxqi
Dear CCP4BBs, I post the question for one of the staff in our lab: Hi everyone Does anyone have the experience of using Phoenix robot automatically setup the crystal? Which plate the Phoenix could use such as 96-well sitting drop or hanging drop? If available could you also tell me the CAT NO.