Dear All,
I am trying to crystallize a protein with Adenosine. My protein is in 20
mM Tris, 300 mM NaCl and the crystals appear in a condition with 5 percent
PEG8K, 0.1 M Sodium Cacodylate. The protein is incubated with adenosine
for 1/2 hr before setting the drop. The crystals appear right aft
1 949 425 1321 Extension 200
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> From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
dusky dew
> Sent: Friday, May 02, 2014 4:39 AM
> To: CCP4BB@JISCMAIL.AC.UK
> Subjec
phone 1 949 425 1321 Extension 200
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> Fax 1 949 425 1611
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> E-mail b...@hrmail.com
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> Web www.hamptonresearch.com
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> From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
dusky dew
> Sent: Saturday, May 03, 2014 1:29 AM
> To: CCP4BB@JISCMAIL.A
Dear all,
I am trying to reproduce some protein crystals. The protein I am getting
after cutting the his tag is very pure. I am using the reported protein
concentration. The cofactor and EDTA needs to be added externally. The
condition has calcium acetate, peg 4k and sodium acetate buffer.
Unfortun
DEAR ALL
I am trying to solve the structure of a protein DNA complex with molecular
replacement. The resolution in about 2.5 angstrom. The spacegroup is P21.
The search model has about 50% identity and is a dimer of a dimer. The
problem is the unit cell is huge and so the number of molecules in as
One tips, sometimes the DNA could be organised in "infinite Helix" in the
crystal, you can try to use DNA 2 times longer than you expect especially
if your DNA molecules has over-hang.
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> Nicolas
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> De : CCP4 bulletin board [CCP4BB@
Can you people please tell me how to calculate a composite omit map in ccp4?
Thanks
Madhu
