Hi every,
I'm working with 2 crystal forms of a protein from 2 different
crystallization conditions. Condition 1 has 100mM MgCl2. Condition 2
doesn't. Both are ~2.9 angstrom. The 2 structures are virtually identical
except in condition1, there is a clear positive density surrounded by a Glu
side
look into these more.
Best,
Gao
On Wed, Dec 14, 2011 at 5:45 PM, bie gao wrote:
> Hi every,
>
> I'm working with 2 crystal forms of a protein from 2 different
> crystallization conditions. Condition 1 has 100mM MgCl2. Condition 2
> doesn't. Both are ~2.9 angstr
Dear Colleagues,
I'm collecting a dataset on our recently repaired Rigaku home source.
Crystal diffracts to 2.2A. Indexing seems to be all fine. However, during
integration, I realize Y-Chi2 is increasing constantly (from 2 to 4.5,
almost linear) within 60 degree collection, whereas X-Chi2 stays t
, it can melt unevenly and re-align the pin a few minutes into the run
> (something similar used to happen a lot at one or two beam lines and it
> drove me nuts until I figured out the need to re-align the crystal after the
> initial screening).
>
> Artem
>
> On Wed, Jun 22, 2011
is a consistency of
> observing the unusual Y-Chi2 with that crystal again then it is likey that
> the crystal maybe highly imperfect. If not, then there could have been a one
> time un-nailed problem occurred during that collection.
>
> Cheers,
>
> Shiva
>
> On Thu, Jun
BTW, I've collected a better dataset with reasonable Chi2. Seems like the
first crystal is imperfect.
Thank everyone for your help!
On Mon, Jun 27, 2011 at 7:59 PM, bie gao wrote:
> Jim, that's the first thing I tried - triclinic gave similar Y-Chi2
> profile.
> Shiva, not
Dear all,
Does anyone know how to measure distance between coiled-coil helices? prefer
axis distance, but residue distance is fine too. Thanks!
Jinhua
