[ccp4bb] phase combination

Dear CCP4s I am trying to solve a structure for which I have two datasets: One comes from a selenomethionine derivatized crystal at 3 A resolution and the second comes from a native one that gave 2.6A. I have obtained workable experimental phases by SAD using SOLVE/RESOLVE. With this I have b

[ccp4bb] Se oxidation

Dear AllI would like to know whether oxidation of Se entails any problem for SAD or MAD experiments and/ or how to resolve it. Cannot use DTT or reducing agents in my protein (extracellular and disulphide bonds are important).ThanksDr. R.DepetrisWeill Cornell Medical College ___

[ccp4bb] Ecoli K12 SB536

Hello All: I would like to know if anyone has or knows how to gte this Ecoli strain which proved to be good for periplasmic protein expression. Ecoli K12 SB 536. If not, which other strain is good for that? Thannks Much Dr. R. Depetris Weill Cornell Medical College __

[ccp4bb] Adding a transmembrane segment

Dear All I am working with a viral protein that contains an ectodomain, a TM segment and a cytoplasmic region. I was wondering if there is a general strategy that allows the addition of the transmembrane segment to the ectodomain without compromising solubility and secretion. Thanks Dr. R. D