Re: [ccp4bb] powdery residue on pucks?

2020-02-22 Thread Zachary A. Wood
them. Since doing this, we have also noticed that the pin bases are less prone to oxidation, which is nice (and supports our electrochemistry hypothesis). Maybe give that a try? Best regards, Z *** Zachary A. Wood, Ph.D. Associate Professor and

[ccp4bb] Post-doc opportunity.

2020-03-03 Thread Zachary A. Wood
wards the development of new bio-based plastics and coatings. Best regards, Z *** Zachary A. Wood, Ph.D. Associate Professor and Graduate Coordinator Department of Biochemistry & Molecular Biology University of Georgia Life Sciences Building, Rm

Re: [ccp4bb] Flexible C terminus

2020-03-12 Thread Zachary A. Wood
residue C-terminus that used entropic force to change the structure of the folded portion of the protein to enhance ligand affinity. Best regards, Z *** Zachary A. Wood, Ph.D. Associate Professor and Graduate Coordinator Department of Biochemistry

[ccp4bb] A Max Perutz question

2019-04-10 Thread Zachary A. Wood
it can coil, takes up this configuration spontaneously, as the only one which satisfies the stereochemical requirements of its amino acid sequence.” Thank you for any help you may be able to offer! Best regards, Z *** Zachary A. Wood, Ph.D. Associate

Re: [ccp4bb] A Max Perutz question

2019-04-10 Thread Zachary A. Wood
pointed me to Georgina Ferry’s biography of Max Perutx (Max Perutz and the secret of life). After a quick trip to the library, I now have a much better photo. Best regards, Z *** Zachary A. Wood, Ph.D. Associate Professor and Graduate Coordinator

Re: [ccp4bb] glycosylated density blob?

2017-01-26 Thread Zachary A. Wood
You mentioned that you have PMSF in your crystallization condition…and PMSF does modify serines. Best regards, Z *** Zachary A. Wood, Ph.D. Associate Professor Department of Biochemistry & Molecular Biology Universit

Re: [ccp4bb] Ligand identification in X-ray density

2024-06-05 Thread Zachary A. Wood
kosmotrope). Best regards, Z *** Zachary A. Wood, Ph.D. (He/Him) Professor and Graduate Coordinator Josiah Meigs Distinguished Teaching Professor Associate Director of SER-CAT Department of Biochemistry & Molecular Biology University of Georgia

Re: [ccp4bb] Ligand identification in X-ray density

2024-06-05 Thread Zachary A. Wood
It is pretty well-ordered, so I would expect the H-bonding pattern to distinguish between betaine and MPD. Best regards, Z *** Zachary A. Wood, Ph.D. (He/Him) Professor and Graduate Coordinator Josiah Meigs Distinguished Teaching Professor

Re: [ccp4bb] Ligand identification in X-ray density

2024-06-05 Thread Zachary A. Wood
-583-0304 Lab:706-583-0303 FAX: 706-542-1738 *** From: Jeroen Mesters Date: Wednesday, June 5, 2024 at 1:17 PM To: Zachary A. Wood Cc: CCP4BB@jiscmail.ac.uk Subject: Re: [ccp4bb] Ligand identification in X-ray density You don't often get e