[ccp4bb] highly soluble proteins

2007-08-07 Thread Yvonne Leduc
Our lab is trying to crystallize a highly soluble (100+ mg/ml) protein with a molecular weight of 35 kd. The protein was screened against 1536 conditions at 20 mg/mL. Most drops were either clear or produced "bubbles" (often oily looking). The few that had precipitate contained high concentration

[ccp4bb] SUMMARY: highly soluble proteins

2007-08-08 Thread Yvonne Leduc
Thank you to all who responded. I find it amazing how generously helpful the crystallography community is. Lots of good ideas. Yvonne _ -Original Message- Sent: Tuesday, August 07, 2007 12:17 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb]

Re: [ccp4bb] kendrew model

2007-10-24 Thread Yvonne Leduc
E. Dickerson. Or, as I read it, reprinted in "Present at the Flood" by R.E. Dickerson, 2005. p269-273. Yvonne Leduc U of Saskatchewan Bernhard Rupp a écrit : Dear all, particlarly the history acquainted: does someone perhaps know where the big metal 2x2x2 meter myo model of John