Hi,
I've given a couple talks/demos to HS students. As much as I love enzymes, I
found it more effective to show structures of proteins binding to other
biological molecules (not just other proteins). I typically show
phosphatidylinositol binding protein (PITP) bound to phosphatidylcholine,
My collaborator was successful in purifying soluble rat phosphatidylinositol
transfer protein in E. coli when overexpressing GroEL/ES. They estimated ~50%
of the protein became soluble. We were able to solve the structure from
protein purified using this expression construct. We did not expe
Hi all,
We are preparing to replace a cryosystem on our home rotating anode.
Does anyone have any insight into a comparison of the Oxford Cobra
system and the Rigaku X-treme?
Many thanks,
Marilyn Yoder
Marilyn D. Yoder
Division of Cell Biology & Biophysics
School of Biological Scie
Hello,
I'm having problems adding XYP (beta-xylose) to my glycosylation chain in a
plant protein.
I have a 1.87 Angstrom map, 4 molecules in the asymmetric unit. I have two
primary N-glycosylation sites, per protein. I am not using NCS for refinement
or model building.
The density for the NA
Hi Ting,
What D. Ekiert was so ungraciously saying, is that instructions to unsubscribe
from the bulletin board can be found here:
http://www.ccp4.ac.uk/ccp4/ccp4bb.php
Regards, Marilyn Yoder
Marilyn D. Yoder
School of Biological Sciences
University of Missouri-Kansas City
Kansas City, MO
