[ccp4bb] R-free flag problem.

.refine gives Rree-R label that other program could not recognize. Could anyone please help me to solve this problem? Thank you very much in advance, Best, Vinson, Liang

Re: [ccp4bb] R-free flag problem

Dear all, Thank you very much for your suggestions. I try Pavel's easy method. Hopefully, the result from phenix.model_vs_data would be enough for PDB.   Best wishes,   Vinson     发件人： Pavel Afonine 收件人： PHENIX user mailing list 抄 送： Vinson LIANG 发送日期： 2010/10/22 (周五) 12:

Re: [ccp4bb] R-free flag problem

2, 2010 at 02:33:03PM +0800, Vinson LIANG wrote: > > > > > > Dear all, > > > > > > > > > > Thank you very much for your suggestions. > > > > I try Pavel's easy method. Hopefully, the result from p

Re: [ccp4bb] Strange density on Serine oxygen.

inked glycosylation. All the best, Vinson  发件人： Savvas Savvides 收件人： Vinson LIANG 抄 送： CCP4BB@JISCMAIL.AC.UK 发送日期： 2010/11/24 (周三) 9:27:31 下午 主 题： Re: [ccp4bb] Strange density on Serine oxygen. Hi Vinson Beyond the possibility for another type of residue as al

Re: [ccp4bb] Strange density on Serine oxygen.

s Savvides 收件人： Vinson LIANG 抄 送： CCP4BB@JISCMAIL.AC.UK 发送日期： 2010/11/24 (周三) 9:48:28 下午 主 题： Re: [ccp4bb] Strange density on Serine oxygen. Hi Vinson is O-glycosylation possible at all given the origin of the protein and the expression system used?  best  Savvas On 24 Nov 2010, at 14:42, Vin

Re: [ccp4bb] Strange density on Serine oxygen.

lation > (http://www.cbs.dtu.dk/databases/OGLYCBASE/). > > best regards > Savvas > > > Savvas Savvides > Unit for Structural Biology @ L-ProBE > Ghent University > K.L. Ledeganckstraat 35, 9000 Ghent, Belgium > Ph. +32  (0)472 928 519 http://www.LProBE.ugent.be/xra

[ccp4bb] How to generate lib file for new ligand?

t wishes, Vinson Liang

[ccp4bb] Is it possible to mutate a reversible epimerase into an inreversible one?

Dear all,   Sorry for this silly biochemistory question.  Thing is that I have a reversible epimerase and I want to mutate it into an inreversible one. However, I have been told that the ΔG of a reversible reaction is zero. Which direction the reaction goes depends only on the concentration of

Re: [ccp4bb] Is it possible to mutate a reversible epimerase into an inreversible one?

to break the EQ of the reaction. So I think the best thing I could do is to take Mickael's suggestion and add another enzyme to change the concentration of one substrate.   However, Lijun's comment do open my mind.   I really appreciate all your help,   Best Regards,   Vinson Liang