Calling all Crystallographers, Please Help!
RE: Refining two different covalent species at the same position.
I have a protein that is a homo-tetramer ABCD with two symmetrical active sites
sitting on a two-fold axis that runs through the AB/CD interface (space group
P21212). We developed an in
I am looking for some expert advice on enzyme kinetics but for an assay in
plate format which has the substrate bound. This is different that the usual
homogeneous plate assays or heterogeneous assays where the enzyme (catalyst) is
plate bound.
In short I am trying to look at the Ki of a compo
