[ccp4bb] How to get rid of the skin in the screening drop

2009-09-05 Thread Shu XU
gents, octyl-beta-glucoside in the protein solution, but that didn't help. Is it because my protein is membrane assiociate protein or the hydrophobity of this protein is too high? I do need help and thanks a lot! Yours, Shu Xu

Re: [ccp4bb] How to get rid of the skin in the screening drop

2009-09-06 Thread Shu XU
Lower the concentration did help a little bit. I didn't the 6 mg/ml setups, but still got some skins. You are right the skins are more likely to find in the condition of PEGs...but also find in other conditions. Lower the conc. of PEGs is a good advice, and I'll try it. Thanks. Shu On Sun, Sep 6,

Re: [ccp4bb] How to get rid of the skin in the screening drop

2009-09-07 Thread Shu XU
mineral oil, paraffin oil, silicon oil. That didn't help. Maybe it is time to try the treated ones. Thank you, Patrick. Shu On Mon, Sep 7, 2009 at 6:50 AM, Patrick Shaw Stewart wrote: > Shu XU > > > > Which type of microbatch plates are you using? > > > > I ask

Re: [ccp4bb] [ccp4bb] Symmetry problem

2014-02-19 Thread Shu Xu
, then you can combine this to the other domain. Cheers, Shu --- Shu Xu, Ph.D. Research Fellow Department of Biochemistry Vanderbilt University School of Medicine Tel: (615)-343-7327 E-mail: xushuh...@gmail.com On Feb 19, 2014, at 4:40 AM, herman.schreu