[ccp4bb] Highest shell standards

better to have a good 1.8 Å structure, than a poor 1.637 Å structure." Are these recommendations still valid with maximum likelihood methods? We tend to use more data, especially in terms of the Rmerge and sigma cuttoff. Thanks in advance, Shane Atwell

[ccp4bb] Scientist and intern positions open in crystallization group

We have two positions open in our crystallization group, one for a scientist and one for an intern. Please use our website to apply: http://www.sgxpharma.com/careers/opportunities.php Shane Atwell Associate Director, Crystallization SGX Pharmaceuticals 10505 Roselle Street San Diego, CA 92121

Re: [ccp4bb] bigger size - > better diffraction?

in getting them habituated to the cryo. Shane Atwell > -Original Message- > From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On > Behalf Of Jenny > Sent: Wednesday, April 04, 2007 5:50 AM > To: CCP4BB@JISCMAIL.AC.UK > Subject: [ccp4bb] bigger size - > better diff

[ccp4bb] Nucleotide duplexes by direct methods

A friend of mine asked me whether small nucleotide duplexes can be solved by direct methods. I think their data is 2.0A.

[ccp4bb] Opportunity for beamline technician

skills are necessary, which will be used for interactions inside and outside the company. A commitment to providing data of the highest quality is essential. Availability on nights and weekends will occasionally be required." Shane Atwell SGX Pharmaceuticals

Re: [ccp4bb] Tough ligand to bind?

efore last purification step - at cell lysis - during fermentation - might require readding ligand at various steps Shane Atwell > -Original Message- > From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On > Behalf Of David Briggs > Sent: Thurs

[ccp4bb] Two postdoc positions at SGX Pharmaceuticals

ruct design and protein engineering. Candidates with experience in protein crystallography will have opportunities to solve and refine novel structures." If you are interested please apply on that same web page: http://www.sgxpharma.com/careers/opportunities.php Cheers, Shane Atwell -Origin

[ccp4bb] Crystallization position open at SGX Pharmaceuticals

Research Associate II, Crystallization CRYRA2-08 Research Associate II, Crystallization SGX Pharmaceuticals is an innovative San Diego-based biotechnology company focused on the discovery, development and commercialization of novel, targeted therapeutics directed at addressing unmet medical

[ccp4bb] Packing broke for 2nd molecule in phaser?

I'm attempting a difficult molecular replacement using phaser and low resolution data (3.5A). There's almost certainly only one molecule in the asymmetric unit. Since the entire structure of a homologous multidomain protein doesn't work, I've broken it down in domains and have been attempting to fi

Re: [ccp4bb] Packing broke for 2nd molecule in phaser?

That was "SEARCH ENSEmble mol2 NUM1" at the end. Bad editing. -Original Message- From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Shane Atwell Sent: Friday, June 20, 2008 1:21 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Packing broke for 2nd molecule in ph

Re: [ccp4bb] Packing broke for 2nd molecule in phaser?

Thanks to those of you that replied. The packing bug turns out to have been observed in Phaser 1.3 with ensembles. We upgraded to Phaser 2.1 and the packing problem has disappeared. Thanks Randy. Shane Atwell Director, Crystallization SGX Pharmaceuticals 10505 Roselle Street San Diego, CA

[ccp4bb] difficult MR in R32(H32)

her suggestions? Thanks in advance. Shane Atwell

Re: [ccp4bb] difficult MR in R32(H32)

Thanks Eleanor and everyone else that replied privately. Twinning was a common thought and the data was not by several tests. The MR was solved by getting better data, clean 2.8A vs. the 3.0A I had. With this data phaser was able to solve it easily in R32 with 3 molecules. There was not signifi