Re: [ccp4bb] Measuring protein concentration with 2mM ATP around

2014-01-17 Thread Shae Padrick
tryptophans vs tyrosines your protein contains, and could be sorted out at lower concentration. This should allow rapid detection using a nanodrop instrument. Shae Padrick On Jan 17, 2014, at 8:10 AM, "Cedric Govaerts" wrote: > Dear all, > > We're doing some crystallization

Re: [ccp4bb] fluorescence question

2015-04-23 Thread Shae Padrick
Hi Pascal, Can you give us some details about your experimental setup? If I had to guess, are you using monochrometers set to approximately 2x Trp EX/EM. You may be able to solve this by just adding some cheap colored glass high pass filters to the optical path. Shae Padrick On Apr 23