Dear Will,
if the crystals grow in vapour-diffusion, you can try to overlay the
reservoir with oil to slow down the crystallization process. I know at
least two cases where this technique helped to get plate-shaped crystals
into more 3D shape.
http://scripts.iucr.org/cgi-bin/paper?S00218898960135
Dear Andreas,
maybe the problem is rather rooted in your expression set up. If the
protein was soluble in the small scale test, it is not necessarily the
case in a fermenter run. The parameters may have changed so strongly that
your protein is now expressed mostly insoluble. To my experience, such
