Hi Todd,
You could try dissolving your compound directly into your PEG4000
precipitant. Alternatively, you could try other low molecular weight
PEGs.
Good luck,
Jeff
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of
Green, Todd
Sent: Mon
A follow up to this (for what it's worth) is that it's sometimes easier
to solve a structure by MR from a homologous structure determined by
experimental phases than it is to solve the initial structure by SeMet,
MIR, etc.
An example is the case of the structure determination of the second
isoform
Kendall, All,
One classic example that comes to mind is the multiple binding modes
that have been structurally described for the drug Gleevec AKA Imatinib.
Gleevec or closely related analogs have been shown to bind to the
kinases Abl (1) and c-Kit (2) in a Phe-out conformation of the conserved
Could it be that evaporation on the way from the microscope to the cold
stream increases the effective cryo concentration as well as causing a
beneficial dehydration effect?
There are quite a few publications that have carefully evaluated
optimizing cryo cooling. Check out Elspeth Garman's many e
Dear Brenda,
I'd suggest using a mixture of 75% Paratone-N oil with 25% light, white
mineral oil. Mix the two thoroughly with a positive displacement pipette
then add a small amount next to the mother liquor (ML) drop. Remove a
crystal from the ML using a matched cryo loop (the smaller the better)
